Biological and medical sciences

An introduction to differential scanning calorimetry (DSC)

DSC

Various

Microcalorimetry as a tool for structural biology

Antibody characterization in human blood plasma

GCI

Three SARS-CoV-2 antigens and patient plasma samples (diluted to 5%)

Antibody characterization from COVID-19 patient plasma binding to SARS-COV-2 antigens

Antibodies - thermal stability

DSC

various monoclonal antibodies

Polyol-induced increases in thermal stability of antibodies by DSC

Antibodies - aggregation

DLS

Monoclonal antibodies

Monoclonal Antibody Characterization

Antibodies - monomer or oligomer (method comparison)

DLS

Immunoglobulin G4

Comparing Dynamic Light Scattering and the Analytical Ultra Centrifuge

Antibodies - variable domains

DSC

humanized IgG1 anti-bodies,

Contribution of variable domains to the stability of humanized IgG1 monoclonal antibodies

Bacterial toxins - hydrodynamic changes

SEC

 Adenylate cyclase toxin (CyaA)

Characterizing Calcium-induced hydrodynamic changes using SEC with Triple Detection

Bacterial toxins - oligomeric state

SEC

Anthrolysin (ALO)

Overcoming the negative effect of protein structure on molecular weight measurement by Size Exclusion Chromatography – Two protein case studies with Anthrolysin and Bcl-2

Binding affinity and kinetics – off-rate screening

GCI

Eighty-three (83) crude reaction mixtures (CRM)

Rapid optimization of hit and lead compounds 

Binding affinity and kinetics – kinetic screening

GCI

Collection of small molecules

Molecular interaction analysis by Grating-Coupled interferometry (GCI) 

Disaccharides - lower size limits of measurement

DLS

Sucrose

Measuring Sub Nanometer Sizes Using Dynamic Light Scattering

Disaccharides - lower size limits of measurement

DLS

Sucrose

Confirming the Lower Size Specification of the Zetasizer APS

DNA - melting behavior

DSC

Salmon testes DNA (ST- DNA)

Differential Scanning Calorimetry to measure the binding of ions, water and protons in the unfolding of DNA molecules

Enzymes - concentration

DLS

TAKA Amylase

The Measurement of High Concentration Proteins Using DLS: TAKA Amylase

Enzymes - electrophoretic mobility with metal ions

DLS / ELS

Carbonic anhydrase (CA)

Studying the influence of metal ions on the structural stability of carbonic anhydrase with the Zetasizer Nano ZSP: An orthogonal approach

Enzymes - inhibitor mechanisms

DSC

Kinase

Revealing kinase inhibitor mechanisms: ITC leads the way

Enzymes - monomeric vs oligomeric with pH

DLS

Pyrrolidone carboxyl peptidase (PCP)

Oligomeric states of a thermophile

Enzymes - size exclusion

DLS

Bovine Carbonic Anhydrase

Dynamic Light Scattering as a Size Exclusion Chromatography detector

Enzymes - stability with pH

DLS

Lactase

Using Light scattering to study the stability of Lactase

Globular proteins - size and molecular weight

DLS

Myoglobin, Hemoglobin

Size Measurement and Molecular Weight Estimation of Globins using Dynamic Light Scattering

Hormones - contamination, aggregation

DLS

Insulin, amyloids

Minimizing cross-contamination during automated protein analysis

Hormones - contamination, aggregation

DLS

Insulin, amyloids

Ensuring Accurate and Repeatable measurement of proteins using the Zetasizer APS

Hormones – self-association

TDA

Insulin

Assessing the self-association behavior of Insulin with concentration using Taylor Dispersion Analysis

Immunoglobulin - aggregation

SEC

Immunoglobulin G (IgG)

Characterization of IgG monomers and their aggregates

Immunoglobulin - molecular weight, intrinsic velocity

DLS

sRAGE

Measuring conformational changes of sRAGE with intrinsic viscosity

Immunoglobulin - polydispersity

DLS

Immunoglobulin G

Assessing the quality of proteins using light scattering techniques

Immunoglobulin - size distribution

SEC + DLS

Immunoglobulin G4

Size Exclusion Chromatography Dynamic Light Scattering (SEC-DLS)

Interferons - PEGylation

SEC

Interferon α-5

PEGylated proteins: Optimizing conjugation reactions using triple detection SEC

Large molecules - aggregation

DLS / SEC

Bovine serum albumin (BSA)

Comparison of SEC-LS and DLS capabilities in the detection and quantification of large protein aggregates

Large molecules - monomers and dimers

DLS + SEC

Human Serum Albumin (HSA), Alcohol dehydrogenase (ADH)

A comparison of the resolution and detection sensitivity of SEC-LS and DLS

Lectins - binding strength

DLS

Concanavalin A

Binding strength of sugars to Concanavalin A

mRNA - riboswitch activity

ITC

Bacterial mRNA

Implementation of kinITC into AFFINImeter

Nanobubbles - size and concentration

NTA

Bovine Serum Albumin and Lysozyme contaminants on surfaces

Nanobubble Applications and Characterization by Nanoparticle Tracking Analysis

Nanoparticles - size and concentration

NTA

Oligonucleotide-functionalized gold colloid

Gold Nanoparticle Applications and Characterization by Nanoparticle Tracking Analysis

Nanoparticles - size and concentration

DLS

Ultrasperical Gold Nanoparticles

Measuring the size of gold nanoparticles using multi-angle dynamic light scattering (MADLS)

Polyglucosides - molecular weight

DLS

Dextran

Characterization of Dextran using Light Scattering Techniques

Polyglucosides - molecular weight

DLS

Dextran

Molecular weight determination of polymers and polysaccharides using the Zetasizer Nano

Polysaccharide polymers - molecular weight and structure

SEC

Chitin/Chitosan

Using triple detection GPC/SEC to determine the molecular weight and structure of Chitosans

Polysaccharide polymers - molecular weight distribution

SEC

Chitin/Chitosan

Characterization of chitosan from various sources

Proteins -  melting behavior

XRD

Thyroglobulin

SAXS study on the thermostability of a protein in solution

Proteins  - molecular weight

SEC

Dextran, Bovine Albumin

Protein and polymer molecular size by GPC/SEC

Proteins - agglomeration

ELS

Lysozyme

Zeta potential measurement of Lysozyme in the High Concentration Zeta Potential Cell

Proteins - aggregate size

DLS

Bovine serum albumin (BSA), β-lactoglobulin,

Combining technologies for the superior detection, separation and characterization of protein aggregates

Proteins - aggregates

DLS

Lysozyme

Adaptive Correlation: Better insight to the presence of aggregates

Proteins - aggregation index

DLS

Immunoglobulin G (IgG)

Enhanced aggregate detection: monitoring protein stability using dual angle light scattering

Proteins - aggregation point

DLS

Ovalbumin

Characterisation of protein aggregation point

Proteins - breakdown to polypeptides

SEC

Alcohol dehydrogenase

Typical protein applications for advanced analytical SEC

Proteins - complexation and binding

DLS

Bovine serum albumin

Characterization of protein-polyelectrolyte complexes

Proteins - crystal structure

XRD

Lysozyme

Transmission diffraction. Measurement and unit cell refinement of lysozyme

Proteins - eliminating cross-contamination

DLS

Bovine serum albumin (BSA) and lysozyme,

Demonstrating that the Zetasizer APS eliminates cross contamination

Proteins - hydrodynamic radius

DLS

Lysozyme

Measurements of 0.1mg/ml Lysozyme in 2μl cell volume

Proteins - hydrodynamic radius, concentration

DLS

Bovine serum albumin

Protein measurements with the Zetasizer µV connected to a GE AKTA SEC system

Proteins - hydrodynamic size and zeta potential

ELS

Human Serum Albumin (HSA)

Improving Protein Zeta Potential Measurements Utilizing A Novel Diffusion Barrier technique

Proteins - ligand binding

NTA

Biotin-Streptavidin complex

Determining fluorescence Limit of Detection with Nanoparticle Tracking Analysis (NTA)

Proteins - microrheology

DLS + ELS

Bovine serum albumin

DLS Microrheology on the Zetasizer Nano ZSP/ZS: Use of a zeta potential pre-measurement step to assess tracer particle-sample interactions

Proteins - modeling concentration effects

DLS

Colloidal silica spheres

Using the Zetasizer Nano to study concentration dependent changes in protein size

Proteins - molecular weight

SEC

Bovine serum albumin

Measuring protein molecular weight using the Viscotek SEC-MALS 20 connected to a Waters Empower® SEC system

Proteins - number and size distribution

DLS

Lysozyme

Number and volume size distributions

Proteins - oligomeric state

SEC

Human serum albumin (HAS)

Characterization of Human Serum Albumin using the Viscotek TDAmax

Proteins - particle size and zeta potential

ELS

Human Serum Albumin (HSA)

Measurements of protein electrophoretic mobility using the Zetasizer Nano ZSP

Proteins - particle size with concentration

DLS

Human Serum Albumin (HSA)

The Effect of Concentration on Dynamic Light Scattering Measurements of Proteins

Proteins - particle size, zeta potential, molecular weight

DLS + ELS

Ferritin

Characterization of Ferritin

Proteins - radius of gyration

XRD

Glucose Imerase

ScatterX78 for BioSAXS studies on dilute protein solutions

Proteins - resolution of mixtures

SEC

Glucose Oxidase, Lactoperoxidase, Lactoferrin, and Lysozyme

Analysis of Protein Mixtures with Multi-Detector SEC

Proteins - size, aggregation with temperature

DLS

Lysozyme and bovine serum albumin (BSA)

Enhanced Protein Aggregation Detection Using Dual Angle Dynamic Light Scattering

Proteins - size titration

DLS + ELS

Bovine serum albumin

Automated protein characterization using the MPT-2 Autotitrator*

Proteins - stability in storage

DLS

Immunoglobulin

Effect of storage conditions on Immunoglobulin

Small molecules: kinetic screening with waveRAPID

GCI

Target protein against library of small molecules

Pushing the boundaries in pharmaceutical hit discovery with the novel waveRAPID^® kinetic assay 

Small molecules: large target-to-analyte molecular weight

GCI

Various small molecule inhibitors of Carbonic Anhydrase II (CAII)

Sensitive kinetic analysis of small molecules binding to large drug targets 

Biocompatibility - Interactions, size of complexes

DLS

Keggin ions

Interactions of Bovine Serum Albumin with Aluminum Polyoxocations

Biocompatibility - surfaces, zeta potential

ELS

Polystyrene lattices with a carboxylated surface

Using zeta potential to assess protein adsorption to surfactant coated latex

Biomaterials - ceramics, composition

XRD

Bio-ceramic calcium phosphate coatings

X-ray diffraction in biomedical research - Chemical and biological analysis of bioceramic calcium phosphate coatings for medical purposes

Biomaterials - chemical phase composition, microstructure

XRD

Seashell

A shell exposed. A multi-dimensional investigation using X-ray diffraction and Computed Tomography

Biomaterials - polymers, molecular structure, intrinsic viscosity

SEC

Polylactic Acid (PLA)

Optimization of PLA/PLGA molecular weight and structure to suit final application requirements

Biomaterials - polymers, molecular weight, concentration

GPC

Poly(lactic-co-glycolic acid) (PLGA)

How switching mobile phases can improve your OMNISEC results

Biomaterials - silk fiber, size, hydrodynamic size

DLS

Spider silk fibers

Protein storage and stability analysis using the Zetasizer Nano ZSP

Biomaterials - silk fiber, zeta potential aggregation

ELS

Spider silk fibers

Predicting protein formulation stability

Adeno-Associated Virus (AAV) - size and size distribution

NTA

Adeno-Associated Virus (AAV) labeled with gold nanoparticles

Adeno-Associated Virus titer and aggregation characterization

Bacteria and Viruses - size and size distribution

NTA

Bacteriophage (Staphylococcus aureus) and various virus particles

Total virus titer in minutes

Bacterial virulence factors - complex formation

SEC

ClpP2. ClpP2, ClpP2R213, CyaA

Characterization of Protein-Protein Interactions using triple detection size exclusion chromatography (SEC-TD)

Bacterial virulence factors - hydrodynamic changes

ELS

CyaA

Characterization of hydrodynamic changes upon cation-binding to proteins

Cell culture - protein stability

ELS

Bovine serum albumin (BSA)

Determining protein isoelectric point: A method comparison

Extracellular vesicles - model systems

NTA

Models for extracellular vesicles

NanoSight NTA Concentration Measurement Upgrade

Extracellular vesicles - size and concentration

NTA

Syncytiotrophoblast microvesicles (STBM), antibody (NDOG II)

Exosomes and Microvesicles: Concentration, sizing and phenotyping using NTA

Extracellular vesicles - size and concentration

NTA

Exosomes, urine, SKOV3 cells

Use of NanoSight Sample Assistant to increase throughput and reproducibility in the physical characterization of exosomes

Extracellular vesicles - stability

NTA

Lyophilized exosomes from human urine and plasma

Measuring Exosome Stability with Nanoparticle Tracking Analysis

Lipid Membranes - phase transitions

DSC

Lipid bilayer membranes

Using DSC to characterize thermotropic phase transitions in lipid bilayer membranes

Membrane proteins: binding with peptides

GCI

GPCR and a 725 Da mutated neurotensin

Binding Kinetics of a G-Protein-Coupled receptor

Membrane proteins - synthesis

SEC

Bovine serum albumin (BSA) in n-Dodecyl-B-D-Maltoside (DDM)

Analysis of membrane protein by multi-detector SEC

MHC class I molecules - Amyloid formation

DLS

β-2-microglobulin (b2m)

Using Light Scattering to Track β-2-Microglobulin Amyloid Formation

Non-viral vectors - shape change

DLS

L-Lysine

The Use of Light Scattering to Study the Structure and charge of Dendritic Poly(L-Lysine)

Polycation DNA complexes - polydispersity

DLS

Polycation-DNA complexes

Using dynamic light scattering to investigate conformational changes

Silica nanoparticles - surface charge

DLS + ELS

Monodisperse silica particles, cationic polymer polyethyl­eneimine(PEI), succinic acid, glutaraldehyde + streptavidin, a fluorophore

Electrokinetic Characterization of Functionalized Silica Nanoparticles

Starburst Dendrimers - Zeta potential, size

DLS + ELS

Amino group + amidoamine units

Starburst Dendrimers Automated Isoelectric Point Measurements

Cancer - diagnostics

DSC

Blood serum

Differential scanning calorimetry as a diagnostic tool for cancer patients

Cancer - treatments

ITC

Small molecule renin inhibitor

Binding thermodynamics of substituted diaminopyrimidine renin inhibitors

COVID – preventive treatment

GCI

Receptor binding domain of SARS-CoV-2 Spike protein, ACE2 protein

Sybody^® candidates compete for ACE2 binding onto SARS-CoV-2 Spike RBD

Immuno­suppressants - stability, particle size distributions

DLS + ELS +LD

Cyclosporines,

Ophthalmic microemulsions: An orthogonal characterization strategy

Public Health Science - elemental concentrations

XRF

Traces of toxic elements (As, Ba, Cd, Cu, Cr, Hg, Ni, Pb, Zn)

Analysis of biological samples

Toxicology of nanoparticles - particle size

NTA

Human blood plasma, gold nanoparticles

Characterization of nanoparticle size and state in nanotoxicological and ecotoxicological studies using Nanoparticle Tracking Analysis (NTA)