00:00:00 | Welcome |
00:00:14 | Introduction |
00:01:51 | Differential scanning calorimetry: Robust and powerful physical characterization of therapeutic protein products |
00:02:29 | Outline |
00:03:25 | Applications of DSC in the Development of Therapeutic Protein Products |
00:04:44 | Untitled |
00:06:22 | DSC Measures Effects of Both Conformational and Colloidal Stabilities |
00:08:21 | Applications of DSC in the Development of Therapeutic Protein Products |
00:08:33 | Untitled |
00:10:05 | Untitled |
00:11:37 | Applications of DSC in the Development of Therapeutic Protein Products |
00:11:55 | Untitled |
00:13:15 | Untitled |
00:14:39 | Applications of DSC in the Development of Therapeutic Protein Products |
00:14:51 | Untitled |
00:15:51 | Applications of DSC in the Development of Therapeutic Protein Products |
00:15:55 | Untitled |
00:16:58 | Applications of DSC in the Development of Therapeutic Protein Products |
00:17:05 | Untitled |
00:19:18 | Applications of DSC in the Development of Therapeutic Protein Products |
00:19:27 | Untitled |
00:23:15 | Neupogen and Zarxio dialyzed into Neupogen formulation |
00:23:26 | Untitled |
00:23:44 | Neupogen and Zarxio dialyzed into Neupogen formulation |
00:24:00 | Applications of DSC in the Development of Therapeutic Protein Products |
00:24:15 | Antibody-Drug Conjugates: A New Class Of Therapeutic Agents |
00:25:00 | Types of Reactions for Synthesizing Trastuzumab-ADC’s (T-DM1) |
00:26:06 | Thermal Stability using Differential Scanning Calorimetry |
00:27:31 | Data are examples of the highly reproducible results with DSC |
00:29:13 | Heating with fluorescence spectroscopy |
00:30:03 | Heating with Far-UV CD spectroscopy |
00:30:34 | Increasing DSC sample throughput with relatively fast heating rates |
00:32:07 | Effect of pH on Avastin Stability |
00:33:37 | Effect of pH on Avastin Stability Observed at all Scan Rates |
00:34:38 | Differential Scanning Calorimetry: Scan Rate Dependence on apparent Tm of Herceptin®, Kadcyla® and Syn-ADC |
00:34:55 | Untitled |
00:35:45 | Plot of 1 𝑇 𝑚 vs ln 𝑣 𝑇 𝑚 2 and Energies of Activation |
00:36:38 | High Throughput Analysis with Automated DSC |
00:38:50 | High Throughput Analysis with Automated DSC |
00:39:20 | 2017 Colorado Protein Stability Conference |
00:41:11 | Thank you for your attentionAny questions? |
00:46:28 | Contact Information |
Differential scanning calorimetry (DSC) is a robust and powerful physical method for characterizing the thermal stability of proteins. During the life history of a commercial therapeutic protein product, DSC analysis is uniquely valuable from early discovery research to commercial product management. Areas in which DSC is used include: comparing stability of potential drug candidates; conducting preformulation and formulation screening studies; biophysical characterization of drug product for regulatory filings; comparability studies after a manufacturing change; and comparing a biosimilar to an innovator drug product. DSC analysis of a protein also can provide insightful data in investigations of the roles of specific domains in the instability and aggregation of multidomain proteins.
In this webinar, some examples of these applications will be described. In addition, results from studies on the effects of heating rate will presented, which demonstrate that faster heating results in both increased sample throughput and increased method sensitivity. Finally, data will be presented from a recent biophysical comparison of antibody drug conjugates to the parent antibody. These results documented that important differences in thermal stability could readily be resolved and quantified with DSC; whereas during heating studies with various spectroscopic techniques no differences were discernable.
In this webinar, some examples of these applications will be described. In addition, results from studies on the effects of heating rate will presented, which demonstrate that faster heating results in both increased sample throughput and increased method sensitivity. Finally, data will be presented from a recent biophysical comparison of antibody drug conjugates to the parent antibody. These results documented that important differences in thermal stability could readily be resolved and quantified with DSC; whereas during heating studies with various spectroscopic techniques no differences were discernable.