| 00:00:00 | Additional Detection Methods for Conventional Calibration |
| 00:01:09 | Additional Detection Methods for Conventional Calibration |
| 00:01:31 | Contents |
| 00:02:16 | INTRODUCTION TO GPC & SEC |
| 00:02:23 | Gel Permeation and Size Exclusion Chromatography |
| 00:03:34 | Expansion of an existing LC system |
| 00:04:48 | Hardware Schematic Conventional GPC |
| 00:05:34 | Conventional Calibration |
| 00:06:46 | Conventional Calibration – Calibration Curve |
| 00:08:06 | Conventional Calibration – Protein Results |
| 00:09:45 | Conventional Calibration |
| 00:10:50 | Overlay of Conventional Calibration Curves |
| 00:11:21 | Conventional Calibration |
| 00:13:04 | LIGHT SCATTERING DETECTION |
| 00:13:19 | Light Scattering |
| 00:13:51 | Principles of Light Scattering |
| 00:14:40 | Principles of Light Scattering |
| 00:14:58 | Angular Dependence |
| 00:16:35 | Light Scattering Detection |
| 00:18:35 | Light Scattering Detectors |
| 00:19:53 | Light Scattering Detectors |
| 00:21:05 | Zetasizer μV optical setup |
| 00:21:40 | OmniFACE |
| 00:22:18 | Zetasizer for batch and SEC Measurements |
| 00:23:39 | BSA chromatogram |
| 00:24:34 | Conventional Calibration – Protein Results |
| 00:25:55 | VISCOSITY DETECTION |
| 00:26:06 | Viscosity Detector |
| 00:26:36 | Universal Calibration |
| 00:28:05 | Universal Calibration |
| 00:28:32 | Relation Between Structure and Intrinsic Viscosity |
| 00:29:31 | Protein Conformational Changes |
| 00:31:28 | Viscosity Detector |
| 00:31:49 | Conventional Calibration – The issue |
| 00:32:06 | Universal Calibration |
| 00:32:44 | Universal Calibration Line |
| 00:32:54 | Universal Calibration |
| 00:33:14 | Protein Mark-Houwink Plot |
| 00:34:20 | Viscosity Detection |
| 00:34:49 | Viscosity Detectors |
| 00:36:08 | CONNECTION TO AN LC SYSTEM |
| 00:36:21 | Connection of Additional Detectors (270 Detector) |
| 00:39:02 | Connection of Additional Detectors (Zetasizer µV) |
| 00:40:35 | Summary |
| 00:41:49 | QUESTIONS? |
| 00:42:17 | Contact Information |
| 00:42:39 | Additional Detection Methods for Conventional Calibration |
Conventional calibration for proteins in SEC is the most popular method, however the integration of other detectors can give higher efficiency and be more accurate. These detectors can be integrated into your existing protein separation system