| 00:00:00 | Welcome |
| 00:02:12 | How to Gain Insights into Complex Modes of Interaction with ITC |
| 00:03:20 | Overview |
| 00:04:13 | Isothermal Titration Calorimetry: Standard Models vs. Nonstandard Models |
| 00:04:16 | ITC |
| 00:05:20 | ITC |
| 00:07:09 | ITC's Black Legend |
| 00:08:10 | Standard Model Example |
| 00:09:35 | Standard Model Example 2 |
| 00:10:44 | Nonstandard Model Example |
| 00:11:34 | Nonstandard Model Example 2 |
| 00:12:25 | Nonstandard Model Example |
| 00:13:06 | Nonstandard Model Example 2 |
| 00:13:51 | Nonstandard Model: Homotropy |
| 00:15:25 | Nonstandard Model: Homotropy |
| 00:16:17 | Nonstandard Model: Homotropy |
| 00:16:50 | Nonstandard Model: Homotropy |
| 00:18:56 | Nonstandard Model: Homotropy |
| 00:19:47 | Nonstandard Model: Homotropy |
| 00:20:45 | Nonstandard Model: Homotropy |
| 00:22:47 | Nonstandard Model: Homotropy |
| 00:23:26 | Nonstandard Model: Heterotropy |
| 00:24:39 | Nonstandard Model: Heterotropy |
| 00:25:54 | Nonstandard Model: Heterotropy |
| 00:27:17 | Competitive Binding |
| 00:28:43 | Cooperative Binding |
| 00:29:55 | Analytical Tools to Gain Insights into Complex Modes of Interactions with ITC |
| 00:30:27 | Heterogeneous Mixtures of Ligands |
| 00:32:22 | In the Mix: Simultaneous Affinity |
| 00:32:43 | Direct Titration |
| 00:35:25 | The Reaction Builder |
| 00:35:31 | Reaction Builder Example |
| 00:36:00 | Reaction Builder Example 2 |
| 00:36:09 | New Model |
| 00:36:25 | New Model |
| 00:36:41 | New Model |
| 00:37:12 | ITC Isotherms |
| 00:39:43 | Global Fitting |
| 00:40:59 | Global Fitting Results |
| 00:41:23 | The Species Distribution Plot |
| 00:43:40 | Heterogeneous Mixtures of Ligands |
| 00:44:24 | Heterogeneous Mixtures of Ligands |
| 00:44:49 | Experimental Setup |
| 00:47:02 | Application in the Pharmaceutical Industry |
| 00:48:19 | Multiple Site Ligand Binding |
| 00:49:41 | Multiple Site Ligand Binding |
| 00:50:43 | Multiple Site Ligand Binding |
| 00:51:26 | Are S1 and S2 of CaM Independent? |
| 00:52:36 | Are S1 and S2 of CaM Independent? |
| 00:53:08 | Global Fitting |
| 00:54:09 | How to Gain Insights into Complex Modes of Interaction with ITC? |
| 00:55:09 | Thank you for your attentionAny questions?Please type your questions in using the Q&A panel on the right side of your screen |
| 00:55:27 | Contact Information |
| 01:01:25 | Find out more |
Watch our webinar on advanced Isothermal Titration Calorimetry (ITC) data analysis with guest presenters from ITC analysis software providers, AFFINImeter. Together we show how the latest advances in the field of ITC data analysis enable users to “squeeze” the ITC isotherm(s) to get more information than just thermodynamic data and to expand the range of applications of ITC. This webinar is very helpful for ITC users studying complex interactions, who are having difficulties with their data analysis, or simply want to take their analysis to the next level. It is also relevant for researchers from academia and industry working in biomedical applications, particularly in the area of drug design.