00:00:00 | Welcome |
00:01:46 | Getting the most our of your EV NTA measurements:Simple methods for dilution, concentration, and sizing. |
00:02:12 | NTA for EVs Provides: |
00:03:53 | What I’ll share today |
00:04:50 | Optical Arrangement for NanoSight Instruments |
00:05:43 | Particles are Visualized, not imaged |
00:05:43 | Sizing: Stokes-Einstein |
00:05:43 | Scatter Phenomena: Raleigh Scattering |
00:06:47 | Sample Preparation |
00:09:20 | Dilution- Example of appropriate concentration |
00:09:20 | Dilution- Example of over concentration |
00:09:20 | Dilution- Example of under concentration |
00:09:20 | Takeaway on dilution and concentration |
00:10:13 | Within the appropriate dilution range, concentration is not related to the realized mode of the distribution. |
00:12:49 | Camera level and Detection Threshold |
00:13:23 | Choosing a camera level |
00:14:38 | Choosing the camera level |
00:16:35 | What is Detection Threshold (DT) |
00:17:14 | DT 1 and 2 are almost always too low |
00:18:21 | With DT 9 the noise tracking is eliminated |
00:18:51 | Detection Threshold |
00:20:31 | Experimental Design considerations |
00:21:32 | Discussion |
00:43:21 | Contact Details |
In this webinar we discuss techniques for producing high quality size distribution and concentration measurements for extracellular vesicle preparations with NanoSight. For characterizing the size and concentration of EVs the focus will be on understanding sources of background particles, proper dilution methods, and design of experiments. The influence of camera and detection settings on the results will be discussed in detail, with particular attention to understanding the impact on your EV measurements. This webinar will leave a user feeling comfortable with measurement of EV samples using NanoSight equipment.