| 00:00:00 | Characterization of the Size and Molecular Weight and Oligomeric State of Proteins using the Malvern Zetasizer Systems in batch and flow modes |
| 00:01:47 | Characterization of the size, molecular weight and oligomeric state of proteins using the Malvern Zetasizer systems in batch and flow modes |
| 00:02:51 | Abstract |
| 00:03:01 | Contents |
| 00:04:11 | Introduction to Theory and Instruments |
| 00:04:17 | Introduction |
| 00:05:31 | Introduction |
| 00:06:49 | Introduction |
| 00:08:31 | Introduction |
| 00:09:45 | Introduction |
| 00:10:59 | Introduction |
| 00:11:56 | Electrophoretic Light Scattering |
| 00:13:10 | Comparison of Zetasizer Models |
| 00:13:31 | Malvern Zetasizer Nano ZS90 |
| 00:14:49 | Malvern Zetasizer Nano ZS |
| 00:16:32 | Nano Zetasizer Nano ZSP |
| 00:17:34 | Malvern Zetasizer µV |
| 00:18:54 | Malvern Zetasizer APS |
| 00:20:17 | Model Summary |
| 00:21:13 | Example Results |
| 00:21:30 | The Proteins being Studied |
| 00:23:51 | Protein Analysis in Batch Mode |
| 00:24:15 | DLS in Batch Mode |
| 00:25:13 | DLS in Batch Mode |
| 00:27:02 | DLS in Batch Mode |
| 00:28:25 | DLS in Batch Mode |
| 00:29:50 | DLS in Batch Mode |
| 00:30:23 | SLS in Batch Mode |
| 00:30:51 | SLS in Batch Mode |
| 00:31:19 | SLS in Batch Mode |
| 00:32:34 | Protein Analysis in Flow Mode |
| 00:33:48 | Flow Mode SLS and DLS |
| 00:35:11 | Flow Mode SLS and DLS |
| 00:36:48 | Flow Mode SLS and DLS |
| 00:37:48 | Flow Mode SLS and DLS |
| 00:38:17 | Flow Mode SLS and DLS |
| 00:39:01 | Flow Mode SLS and DLS |
| 00:39:30 | Electrophoretic Light Scattering |
| 00:40:42 | Human Serum Albumin mobility at pH 7 |
| 00:41:45 | Human Serum Albumin mobility at pH 4 |
| 00:42:40 | BSA pH trend |
| 00:43:01 | Microrheology |
| 00:44:08 | Microrheology applications – protein solution viscosity |
| 00:44:55 | Microrheology applications – protein solution viscosity |
| 00:45:36 | Microrheology applications – protein aggregation |
| 00:46:45 | Model Summary |
| 00:47:36 | Questions and Further Information |
| 00:51:13 | Contact Information |
This presentation will discuss the use of the Zetasizer Nano and Zetasizer µV systems for the measurement of the size, molecular weight and oligomeric state of proteins in solution, and the use of these systems as chromatography detectors