| 00:00:00 | Welcome |
| 00:01:55 | Fluorescence Masterclass- |
| 00:02:18 | This presentation discusses |
| 00:02:47 | Why consider fluorescence? |
| 00:03:24 | Introduction to the fluorescence process and properties |
| 00:03:29 | Fluorescence Process |
| 00:04:11 | Fluorescence Spectra |
| 00:04:52 | Fluorescence Spectra |
| 00:05:03 | Fluorescence Spectra |
| 00:05:23 | Fluorescence Spectra |
| 00:06:05 | Types of fluorophore |
| 00:06:15 | Types of Fluorophore |
| 00:07:24 | Types of Fluorophore |
| 00:08:51 | NanoSight instruments for detecting fluorescence |
| 00:08:57 | Fluorescent Measurement |
| 00:09:46 | NanoSight Range for Detecting Fluorescence |
| 00:10:36 | Fluorescence Options Available |
| 00:11:15 | NanoSight Range for Detecting Fluorescence |
| 00:12:09 | Choosing appropriate fluorophores for your laser wavelength |
| 00:13:21 | Choosing appropriate fluorophores for your laser wavelength |
| 00:13:53 | Options for generating fluorescent samples |
| 00:14:01 | Generating Fluorescent nanoparticles |
| 00:15:10 | Structure-specific fluorescent tags |
| 00:17:04 | Direct, specific tags (1) |
| 00:17:51 | Direct, specific tags (2) |
| 00:19:34 | 2-step fluorescent labelling (1o + 2oF) |
| 00:20:44 | 2-step fluorescent labelling (1o + 2oF) |
| 00:21:29 | Points to remember! |
| 00:22:22 | Examples of fluorophores used with NanoSight systems |
| 00:22:28 | Example: Titration of F |
| 00:23:39 | Example: Titration of F |
| 00:24:33 | Example: Titration of F |
| 00:25:37 | Case Study: FBS (Fetal Bovine Serum) |
| 00:26:10 | Sample viewed before optical filter |
| 00:26:10 | Sample viewed after optical filter |
| 00:26:10 | Sample viewed before optical filter |
| 00:27:27 | Sample viewed after optical filter |
| 00:28:38 | Membrane labelling - visual comparison |
| 00:29:23 | Improved labelling with assay development |
| 00:30:32 | 2-step fluorescent labelling (1o + 2oF) |
| 00:31:36 | Some Fluorophores used with NanoSight systems |
| 00:40:15 | This Introductory Fluorescence Master class …… |
| 00:41:01 | Thank you for your attentionAny questions?Please type your questions in using the Q&A panel on the right side of your screen |
| 00:41:01 | Contact Information |
Fluorescence is increasingly considered as a tool within nanoparticle characterisation. The ability to positively identify particular subsets of nanoparticles within a sample is especially important for applications in life science, drug delivery, virology and exosomes’ research. In this introductory webinar we will first explain the fluorescence process and discuss the properties of fluorophores that you need to consider when choosing a label for your nanoparticles. Next we will briefly discuss some of the common approaches that have been used to produce fluorescently labelled nanoparticles. We will explain the “fluorescent mode” available with NanoSight instruments. And finally we give you a taste of some fluorescent nanoparticles that have been characterised with Nanoparticle Tracking Analysis. There will also be time to ask any questions that you have about getting started with fluorescent nanoparticle characterisation with your NanoSight instrument.