| 00:00:00 | Nanoparticle tracking analysis (NTA) |
| 00:02:58 | Nanoparticle Tracking Analysis (NTA) – A New View of Nanoparticle Characterization |
| 00:03:59 | Agenda |
| 00:05:04 | NanoSight history |
| 00:07:42 | NTA - Direct Visualization of Nanoparticles |
| 00:09:06 | Polydisperse TiO2 in water |
| 00:10:10 | Optical Arrangement for NanoSight Instruments |
| 00:12:38 | Principle of Measurement |
| 00:13:14 | NTA Sizing… is an Absolute Method |
| 00:14:58 | Nanoparticle Tracking Analysis |
| 00:16:28 | Particle Sizing in action - Software Analysis |
| 00:17:09 | Software process 23 |
| 00:18:08 | NTA detection limits |
| 00:21:16 | Dynamic Light Scattering |
| 00:21:27 | Dynamic Light Scattering (DLS) |
| 00:22:20 | Principles of DLS |
| 00:23:57 | Limitations of DLS (where NTA can help) |
| 00:25:45 | Comparison between DLS and NTA |
| 00:25:49 | NTA vs. DLS – Monodisperse Result |
| 00:26:27 | Polystyrene Latex Results with Three Techniques |
| 00:27:06 | NTA vs. DLS – Bimodal Sample |
| 00:28:05 | 100+200 |
| 00:28:35 | NTA vs DLS - Bimodal Sample |
| 00:29:42 | PSL Mixtures |
| 00:30:41 | Spike... |
| 00:31:43 | Additional Capabilities of NTA |
| 00:31:49 | Particles are Counted and Concentration is Measured |
| 00:34:22 | Concentration Measurement Linearity |
| 00:35:08 | Size vs. Intensity vs. Number |
| 00:36:56 | Scattered Intensity - An Additional Variable |
| 00:37:01 | Separate Populations or Statistical Noise? |
| 00:37:34 | Resolving Mixtures of Different Particle Types |
| 00:38:06 | Fluorescent Mode Option |
| 00:38:54 | Detection of Fluorescent Particles in Mixtures |
| 00:39:29 | Analysis of 100 nm Fluorescent Particles in FBS |
| 00:40:19 | FBS no filter |
| 00:40:38 | FBS. 100nm fluro. green laser. 60 sec. With filter |
| 00:41:14 | NTA devices worldwide |
| 00:41:35 | Where would you want to add NTA information to DLS? |
| 00:41:39 | Polydisperse distributions |
| 00:42:48 | Tails of the distribution |
| 00:43:51 | When ‘a number’ won’t adequately describe your sample |
| 00:44:40 | To learn more about the NanoSight range: |
| 00:56:39 | Contact Information |
The addition of the NanoSight range of nanoparticle characterization instruments adds another orthogonal technique to the suite of tools available to the particle scientist. Nanoparticle Tracking Analysis (NTA) is a unique and patented technology that enables rapid and simultaneous multi-parameter analysis and characterization of many different types of nanoscale particles. With the ability to visualize individual particles in solution, then analyze for size and count/concentration, both under scatter and fluorescence mode, NanoSight systems are closely aligned with Malvern’s market-leading Zetasizer range, extending Malvern’s solutions for those working at the nanoscale. This webinar will serve as an introduction to the technique for Malvern’s Zetasizer users. The basics of the science will be covered, with numerous examples and comparison to existing Zetasizer capabilities and results. NanoSight’s unique technology has become accepted in laboratories around the world and is widely cited in research publications. With rapid advances in nanomaterials and the life sciences, in biopharmaceuticals especially, the demand for new technologies to answer continually evolving analytical challenges has never been greater. This is a very exciting time and by bringing together complementary, leading edge technologies, Malvern continues to focus on delivering the analytical tools which laboratories so urgently need.