| 00:00:00 | A new SEC detector for proteins – The Zetasizer µV |
| 00:01:15 | A new SEC detector for proteins – The Zetasizer µV |
| 00:01:58 | Outline |
| 00:02:42 | Dynamic Light Scattering – Particle Size |
| 00:06:19 | Static Light Scattering – Molecular Weight |
| 00:08:09 | Combined size and molecular weight measurements in a single instrument |
| 00:08:42 | Zetasizer µV features & specs |
| 00:09:37 | Zetasizer µV as a SEC detector (SEC-LS) |
| 00:11:41 | Zetasizer μV optical setup |
| 00:13:21 | OmniFACE and OmniSEC |
| 00:14:36 | Zetasizer μV for batch and SEC measurements |
| 00:16:29 | Batch DLS applications |
| 00:19:01 | Effect of Storage Conditions |
| 00:23:01 | Ligand binding |
| 00:25:10 | Zetasizer µV SEC-LS applications |
| 00:27:09 | SEC-LS measurements |
| 00:28:30 | Correlograms |
| 00:30:07 | BSA chromatogram |
| 00:31:49 | Trypsin Inhibitor |
| 00:32:39 | BSA in batch and flow |
| 00:33:52 | Beta-lactoglobulin |
| 00:35:20 | Beta-lactoglobulin |
| 00:37:33 | Summary |
| 00:38:30 | Thanks for listening Any questions? |
| 00:40:28 | Contact Information |
The Zetasizer μV, an established batch DLS instrument, can now be used as an SEC detector to yield molecular weight and hydrodynamic radius for any SEC experiment. The theory of operation and connection to a system will be described