| 00:00:00 | Zetasizer Nano Applications |
| 00:00:05 | Outline |
| 00:00:19 | Light Scattering |
| 00:00:43 | Static Light Scattering (SLS) |
| 00:00:55 | Dynamic Light Scattering (DLS) |
| 00:01:09 | Protein Structure & Function |
| 00:01:39 | Thermal Denaturation |
| 00:01:54 | Protein Melting Point (TM) |
| 00:02:19 | Protein Thermal Stability |
| 00:02:38 | Shelf Life Correlation |
| 00:02:59 | Zetasizer Technical Specifications |
| 00:03:10 | Why Malvern Instruments? |
The Zetasizer Nano, using dynamic light scattering, can monitor the thermal denaturation of proteins leading to irreversible loss of structure and function as the protein unfolds, and the effects of modifications such as glycosylation. When DLS is observed versus temperature, the transition point where aggregation begins to occur is typically lower than the melting point, and is therefore called the aggregation point, or aggregation onset point.