| 00:00:00 | Introduction |
| 00:09:02 | What is a particle's size |
| 00:10:33 | Different particle size methods |
| 00:21:03 | Theory of DLS - dynamic light scattering |
| 00:22:41 | What DLS measures |
| 00:23:47 | How DLS is measured |
| 00:40:44 | DLS application on vaccine stability research |
| 00:49:47 | Q & A |
Dynamic Light Scattering (DLS), sometimes referred to as Photon Correlation Spectroscopy or Quasi-Elastic Light Scattering, is a technique classically used for measuring the size of particles typically in the sub-micron region, dispersed in a liquid.
DLS is typically used for measuring particle size of sub-micron particles. It is adopted across various industries. For instance in Vaccines or bio-formulations this is used in research and development, formulation development, process monitoring, quality control, stability studies, dissolution kinetics, batch release, and other stages in the life-cycle of such medicines in the pharmaceuticals/ bio-pharmaceutical / biologics industry.
In vaccine and bio-formulations, understanding the stability of biological molecules is critical towards ensuring their therapeutic efficacy and immunogenicity. Unstable biological molecules tend to undergo denaturation and aggregation. Influencing factors can be molecule concentration or the level of agitation. Hence it is important to carefully monitor the molecules throughout the development pipeline; from the point of R and D, formulation development, process monitoring to the point of batch release.
The sensitivity of some modern systems is such that it can also now be used to measure the size of macromolecules in solution. In traditional DLS experiments, the scattered light is detected at a single angle and then auto-correlated to determine the diffusion rate of the particles, and ultimately the particle size distribution. Since the direction and the number of photons scattered depends on the size of the particles, for mixed particle sizes a single angle result may misrepresent the true particle size population. Multi-angle dynamic light scattering (MADLS) overcomes many of these drawbacks by automatically combining correlograms from multiple measurement angles to give a robust, angular independent result, with improved resolution, which is suitable for comparison with data from orthogonal techniques.
This webinar is led by Dr Alex van Herk, Principal Scientist at the A*STAR Institute of Chemical and Engineering Sciences (Singapore) and Dr Anand Tadas, Malvern Panalytical's regional application specialist who has a wealth of knowledge in biologicals and DLS. Dr Alex will give an introduction to particle size characterisation and the various methodologies that analysts can engage. Dr Anand will elaborate further into nanoparticle research using dynamic light scattering, with applications towards vaccines among numerous other materials. Interested to improve your R and D / manufacturing process and gain more knowledge about the applications of particle size distribution using DLS?
Join our free series of webinars:
- Webinar 1: Intro to Dynamic Light Scattering and its applications towards vaccine research, formulation and development
- Webinar 2: Recognising good vs bad DLS data: practical tips on sample preparation and handling, software navigation and analysis. More info
- Webinar 3: Nanomedicine involving complex composition and the criteria for using nanoparticle tracking analysis. More info
- Webinar 4: Research applications on gene therapy and virus research using NTA. More info
- Webinar 5: Focus on Vaccine Development 1: Value of DSC as a Complimentary and Insightful Technique for Structural Characterization of a Multi-Domain Protein Antigen. More info
- Webinar 6: Focus on Vaccine Development 2: How Stable is Stable? Combining biophysical techniques and advanced kinetics to support formulation development. More info
- Webinar 7: Strengthening the analytical workflow for rAAV viral vector development. More info
presentadores
Dr Alex van Herk, Principal Scientist II at the A*STAR Institute of Chemical and Engineering Sciences
Dr Alex has been working with the A*STAR Institute of Chemical Engineering Sciences in Singapore since 2012. Prior to that he was the emeritus professor in Polymer Reaction Engineering at the Eindhoven University of Technology, the Netherlands (where he worked full-time from 1986 till 2012). He also gives trainings to industry. His field of research is nanotechnology, water-based coatings and emulsion polymerization. He is editor of four books and author of 200+ papers.
He is a leading specialist in emulsion polymerization/nanotechnology with application areas in capsules for controlled release, smart coatings and marine antifouling.
Dr Anand Tadas , Regional Technical Specialist at Malvern Panalytical
Dr Tadas heen associated with Malven Panalytical for more than 10 years. He specializes in the Nanometrics product ranges. Anand received his Ph.D. in Physical Chemistry (colloidal science) from Mumbai India. He is a holder of 3 patents on inert metal processing which are licensed. He has also guided 4 students for their Masters (M.Tech) programmes. At present, Dr Tadas is focusing on using the orthogonal characterization of materials particularly in the delivery applications across different sectors.
Preguntas más frecuentes
Why should I attend ? What will I learn ?
1. How Dynamic Light Scattering technology works
2. What are its limitations
3. How the traditional limitations of DLS can be mitigated using MADLS and Adaptive correlation
How long is this webinar?
40 minutes is the intended speaker time with additional time for addressing queries.
Más información
Join our free series of webinars: "Vaccines and biopharma related research: Improving your data analysis, expanding your research applications"
- Webinar 1: Intro to Dynamic Light Scattering and its applications towards vaccine research, formulation and development
- Webinar 2: Recognising good vs bad DLS data: practical tips on sample preparation and handling, software navigation and analysis. More info
- Webinar 3: Nanomedicine involving complex composition and the criteria for using nanoparticle tracking analysis. More info
- Webinar 4: Research applications on gene therapy and virus research using NTA. More info
- Webinar 5: Focus on Vaccine Development 1: Value of DSC as a Complimentary and Insightful Technique for Structural Characterization of a Multi-Domain Protein Antigen. More info
- Webinar 6: Focus on Vaccine Development 2: How Stable is Stable? Combining biophysical techniques and advanced kinetics to support formulation development. More info
- Webinar 7: Strengthening the analytical workflow for rAAV viral vector development. More info