| 00:00:00 | Polymer Solution Characterisation - Part 1. "Absolute" molecular weight - fact or fiction? |
| 00:04:45 | Absolute molecular weight – fact or fiction? |
| 00:06:11 | Polymer is a composite of the Greek words poly and meros, meaning “many parts.” |
| 00:07:04 | Molecular weight |
| 00:08:10 | Polymer molecular weight - Polystyrene |
| 00:08:54 | Molecular weight |
| 00:09:39 | Molecular weight |
| 00:11:30 | Classical Determination of MW |
| 00:13:08 | Osmometry |
| 00:15:24 | End group analysis |
| 00:16:43 | Viscosity |
| 00:18:41 | Other Methods for Molecular Weight Determination |
| 00:20:06 | Light Scattering |
| 00:21:21 | Static light scattering theory |
| 00:22:17 | Anisotropic scatterers and angular dependence |
| 00:23:26 | Accounting for anisotropic scattering |
| 00:25:10 | Right-angle light scattering (RALS) |
| 00:25:48 | Low-angle light scattering |
| 00:25:53 | RALS/LALS |
| 00:26:28 | Multi-angle light scattering |
| 00:27:20 | The 3 approaches in plain English |
| 00:28:10 | Batch vs GPC/SEC measurement |
| 00:29:48 | Batch measurements |
| 00:31:26 | GPC/SEC - The beginnings of the technique |
| 00:34:06 | GPC/SEC - Detection and Calibration strategies(1) Conventional Calibration |
| 00:35:37 | GPC/SEC - Detection and Calibration strategies |
| 00:36:56 | GPC/SEC - Detection and Calibration strategies(2) Universal Calibration |
| 00:38:32 | Detection and Calibration strategies(3) Light Scattering Calibration |
| 00:39:32 | Absolute molecular weight? |
| 00:40:28 | GPC/SEC and GPC/SEC with Light Scattering |
| 00:42:36 | Absolute molecular weight? |
| 00:52:10 | Contact Information |
The measurement of the "absolute" molecular weight (or molar mass) of polymers and proteins is often mentioned and referenced but what does it mean? This talk will present a summary of the solution techniques available for measurement of molecular weight and discuss how "absolute" they can be considered to be. The focus will be on light scattering techniques, both in batch mode and when incorporated with chromatography systems.