New Analytical Developments in Protein Size Exclusion Chromatography

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00:00:00 New Analytical Developments in Protein Size Exclusion Chromatography
00:01:08 New Analytical Developments in Protein Size Exclusion Chromatography
00:01:49 Contents
00:03:08 INTRODUCTION TO SEC
00:03:12 Gel Permeation and Size Exclusion Chromatography
00:04:22 Hardware Schematic SEC System
00:07:04 SEC Tetra Detection
00:09:02 Light Scattering
00:09:43 The Principle – Light Scattering
00:10:13 Angular Dependence
00:11:58 Isotropic Scatterers
00:13:06 Viscosity Detector
00:14:27 Relation Between Structure and Intrinsic Viscosity
00:15:52 WHAT IS CALIBRATION?
00:16:12 What is Calibration?
00:18:08 Calibration of Light Scattering Detector
00:19:23 Principles of light scattering
00:20:15 Is light scattering ‘absolute’?
00:21:34 Alternative Calibration Methods
00:23:15 CALIBRATION OF A POLYMER
00:23:27 Calibration of a Polymer in SEC
00:24:16 Calibration of a Polymer in SEC
00:25:20 CALIBRATION OF PROTEINS
00:25:25 Calibration of Proteins in SEC
00:28:50 Current Calibration Standard
00:30:16 Current Calibration Standard
00:30:54 Current Calibration Standard
00:31:06 Current Calibration Standard
00:31:34 Current Calibration Standard
00:31:47 Current Calibration Standard
00:32:52 Current Calibration Standard
00:34:19 Concept
00:34:56 Methods
00:35:53 Methods
00:38:04 Methods
00:40:05 Methods
00:42:56 Methods
00:47:15 Example Calibration Standard - Lysozyme
00:47:40 Example Calibration Standard - Lysozyme
00:49:35 Contact Information
Size exclusion chromatography is a well-established technique for characterization of the size, molecular weight and oligomeric state of proteins. New developments are presented with a focus on calibration methods