| 00:00:00 | Introduction |
| 00:02:14 | New Applications for Zetasizer Nano Series Instrumentation DLS & Zeta Potential |
| 00:02:34 | Characterization of Self-Assembly DNA-binding Surfactants |
| 00:03:12 | Outline |
| 00:03:34 | PART ONE |
| 00:03:39 | DLS and BROWNIAN MOTION |
| 00:04:24 | Dynamic Light Scattering (DLS) |
| 00:05:19 | STOKES-EINSTEIN EQUATION |
| 00:05:47 | MEASURING ELECTROPHORETIC MOBILITY |
| 00:07:31 | PART TWO |
| 00:08:04 | LIPOSOME STRUCTURE |
| 00:09:12 | LIPOSOME STRUCTURE |
| 00:10:11 | CATIONIC LIPOSOMES IN GENE THERAPY |
| 00:11:27 | PNA AMPHIPHILES (PNAA) |
| 00:14:26 | Untitled |
| 00:16:01 | PNAA MICELLE MORPHOLOGY BY SAXS |
| 00:17:15 | PNAA MICELLE SIZE USING ZETASIZER |
| 00:18:40 | TRITON MICELLE MORPHOLOGY BY SAXS |
| 00:19:31 | TRITON MICELLE SIZE USING ZETASIZER |
| 00:20:16 | PNAA Incorporation into Lipid Bilayer |
| 00:22:01 | Size distribution of polar PNA liposomes |
| 00:23:19 | Size distribution of polar PNA liposomes w/DNA |
| 00:24:05 | Mobility distribution of PNAA Lipsomes |
| 00:24:58 | Zeta Potential: DNA adsorption to PNA liposomes |
| 00:27:33 | VALIDATION OF MOBILITY FROM CAPILLARY ZONE ELECTROPHORESIS |
| 00:29:11 | Controlled rupture of liposomes by pH |
| 00:31:49 | CONCLUSIONS |
| 00:32:57 | Contact information |
The Zetasizer Nano is used to characterize novel surfactant systems for DNA separations and gene delivery applications. The application requires a sensitive, low volume, label free method, so the Zetasizer is ideally suited for this