MicroCal VP-DSC Datasheet

Characterizing the stability of proteins and other biomolecules

MicroCal VP-DSC is a sensitive, easy-to-use differential scanning calorimeter for studying samples in solution. It can be used to measure the intramolecular stability of a broad spectrum of biomolecules, including proteins, nucleic acids, lipids, and detergent micellar systems. MicroCal VP-DSC provides fast, accurate transition midpoint (T_m) determination. In addition, a thermodynamic profile can be generated, providing insight into the factors that affect conformation and stability.

Typical applications include determination of protein stability and folding, antibody domain structure determination, characterization of membranes and lipids, and the measurement of ultra-tight molecular interactions.

Figure 1: MicroCal VP-DSC

MicroCal VP-DSC delivers:

• The ability to study molecules in their native state without labeling. Can be used with solutions or turbid suspensions

• The ability to measure very tight binding constants (up to 10²⁰ M^-1)

• Unattended operation after sample loading, which allows you to focus on other tasks

• A complete system without the need for additional accessories, reagents or consumables

Differential scanning calorimetry (DSC) is a powerful analytical tool for characterizing the stability of proteins and other biomolecules. DSC directly measures the enthalpy (ΔH) and temperature (T_m) of thermally induced structural transitions in solution.

DSC data supplies the thermodynamic parameters of protein unfolding, including the midpoint unfolding temperature, enthalpy, entropy, Gibbs free energy, and heat capacity changes upon unfolding.

DSC is also used to determine the change in heat capacity (ΔC_p) of denaturation. DSC can elucidate the factors that contribute to the folding and stability of native biomolecules, including hydrophobic interactions, hydrogen bonding, conformational entropy, and the physical environment.

MicroCal VP-DSC features:

• Nonreactive Tantalum cells for excellent chemical resistance

• Fixed-in-place cells for reproducible ultrasensitive performance with low maintenance

• Three user selectable response times for maximum performance

• User selectable temperature scan rates and range for application versatility

• Self-contained pressurization system (0-45 psi) for studying solutions above their boiling point

• Pressure Perturbation Calorimetry accessory available for determination of partial specific volumes

• Peltier element for precise temperature control

• Includes MicroCal ThermoVac sample preparation and cleaning device

• Controlled by VPViewer software and data analysis performed with Origin

Specifications