| 00:00:00 | Welcome |
| 00:01:24 | Fluorescence Masterclass II- |
| 00:02:03 | This presentation discusses |
| 00:02:40 | NanoSight instruments for detecting fluorescence |
| 00:02:48 | Fluorescence Options Available |
| 00:03:22 | NanoSight Range for Detecting Fluorescence |
| 00:04:17 | Connecting the syringe pump |
| 00:04:22 | Syringe pump connection |
| 00:04:58 | Syringe pump connection |
| 00:05:37 | Syringe pump connection |
| 00:05:59 | Syringe pump flow |
| 00:06:50 | Controls |
| 00:07:18 | Controls |
| 00:08:41 | Example 1 |
| 00:09:03 | Sample information |
| 00:09:44 | Instrument information |
| 00:10:48 | Initial light scatter measurement |
| 00:10:48 | Instrument information |
| 00:11:17 | Measurement in Fluorescent mode |
| 00:11:17 | Instrument information |
| 00:11:38 | Overlay within the software |
| 00:11:38 | Other examples in fluorescence mode |
| 00:11:48 | Low fluorescence/particle |
| 00:11:48 | Fluorescence excess |
| 00:11:48 | Controls |
| 00:12:01 | Buffer |
| 00:12:01 | Non-labelled sample |
| 00:12:01 | Fluorophore alone 1 |
| 00:12:01 | Fluorophore alone 2 |
| 00:12:01 | Quantum dots |
| 00:12:01 | And Finally |
| 00:12:09 | Photobleaching & Airbubbles |
| 00:12:21 | Photobleaching |
| 00:12:21 | Airbubbles |
| 00:12:21 | Summary |
| 00:12:42 | Questions? |
| 00:17:52 | Contact Information |
Fluorescence is becoming an increasingly considered as a tool within nanoparticle characterisation. The ability to positively identify particular subsets of nanoparticles within a sample is especially important for applications in life science, drug delivery, virology and exosomes research. In this second Fluorescence Masterclass we will discuss how to make measurements with NanoSight NTA and how this differs from measurements using light scatter. Next we will discuss which controls are appropriate for your sample. And finally we will help you understand how the visual nature of NTA can aid in the generation of fluorescent nanoparticle.