| 00:00:00 | New Analytical Developments in Protein Size Exclusion Chromatography |
| 00:01:08 | New Analytical Developments in Protein Size Exclusion Chromatography |
| 00:01:49 | Contents |
| 00:03:08 | INTRODUCTION TO SEC |
| 00:03:12 | Gel Permeation and Size Exclusion Chromatography |
| 00:04:22 | Hardware Schematic SEC System |
| 00:07:04 | SEC Tetra Detection |
| 00:09:02 | Light Scattering |
| 00:09:43 | The Principle – Light Scattering |
| 00:10:13 | Angular Dependence |
| 00:11:58 | Isotropic Scatterers |
| 00:13:06 | Viscosity Detector |
| 00:14:27 | Relation Between Structure and Intrinsic Viscosity |
| 00:15:52 | WHAT IS CALIBRATION? |
| 00:16:12 | What is Calibration? |
| 00:18:08 | Calibration of Light Scattering Detector |
| 00:19:23 | Principles of light scattering |
| 00:20:15 | Is light scattering ‘absolute’? |
| 00:21:34 | Alternative Calibration Methods |
| 00:23:15 | CALIBRATION OF A POLYMER |
| 00:23:27 | Calibration of a Polymer in SEC |
| 00:24:16 | Calibration of a Polymer in SEC |
| 00:25:20 | CALIBRATION OF PROTEINS |
| 00:25:25 | Calibration of Proteins in SEC |
| 00:28:50 | Current Calibration Standard |
| 00:30:16 | Current Calibration Standard |
| 00:30:54 | Current Calibration Standard |
| 00:31:06 | Current Calibration Standard |
| 00:31:34 | Current Calibration Standard |
| 00:31:47 | Current Calibration Standard |
| 00:32:52 | Current Calibration Standard |
| 00:34:19 | Concept |
| 00:34:56 | Methods |
| 00:35:53 | Methods |
| 00:38:04 | Methods |
| 00:40:05 | Methods |
| 00:42:56 | Methods |
| 00:47:15 | Example Calibration Standard - Lysozyme |
| 00:47:40 | Example Calibration Standard - Lysozyme |
| 00:49:35 | Contact Information |
Size exclusion chromatography is a well-established technique for characterization of the size, molecular weight and oligomeric state of proteins. New developments are presented with a focus on calibration methods