| 00:00:00 | Live demo of a novel method for protein zeta potential measurement |
| 00:01:46 | Live demo of a novel method for protein zeta potential measurement |
| 00:02:49 | Why protein charge measurement? |
| 00:03:58 | Protein Charge |
| 00:05:19 | Zeta Potential |
| 00:06:11 | Calculating Protein Charge |
| 00:08:10 | Calculating Protein Charge – Methodology |
| 00:09:02 | pH Effects |
| 00:09:53 | Electrophoretic Light Scattering (ELS) |
| 00:10:53 | Electrical considerations |
| 00:11:38 | Volume considerations |
| 00:12:02 | Invasive?? |
| 00:13:56 | What happened? |
| 00:15:03 | What can be done? |
| 00:15:33 | Isolated protein |
| 00:16:30 | Diffusion Barrier Video v2 |
| 00:16:30 | Video demonstration |
| 00:16:49 | Discarding The Rules |
| 00:18:19 | Example Diffusion Barrier ELS Results |
| 00:18:48 | One more pitfall |
| 00:20:26 | Best practices |
| 00:22:34 | Acknowledgements |
| 00:22:48 | Questions? |
| 00:23:05 | Contact Information |
A novel method of measuring the charge of proteins has been developed for the Zetasizer Nano that avoids denaturation, called the diffusion barrier technique. Examples are given and the strengths and limitations of the technique discussed