| 00:00:00 | Protein zeta potential measurements using Malvern’s new diffusion barrier method |
| 00:02:10 | Protein zeta potential measurements using Malvern’s new diffusion barrier method |
| 00:02:22 | Outline |
| 00:02:53 | Zeta Potential (ZP) |
| 00:04:18 | Measuring Zeta Potential |
| 00:05:09 | Zeta potential measurements using micro-electrophoresis |
| 00:06:14 | Laser Doppler Electrophoresis |
| 00:07:12 | Measurement strategy (M3-FFR) |
| 00:08:55 | Henry’s Equation |
| 00:09:35 | Zeta potential measurements of proteins |
| 00:10:10 | Diffusion barrier technique |
| 00:12:15 | Diffusion barrier technique |
| 00:13:54 | HSA measurement – full cell |
| 00:15:20 | HSA measurement – diffusion barrier |
| 00:16:00 | Size distributions before and after measurements |
| 00:16:43 | Important parameters for protein zeta potential |
| 00:17:38 | Investigating MEP using BSA in PBS+1mM DTT(4) Polarisation concentration effects |
| 00:19:08 | Investigating MEP using BSA in PBS+1mM DTT(5) |
| 00:20:42 | HSA in different pH buffers |
| 00:21:00 | HSA sample measured with diffusion barrier |
| 00:21:41 | General recommendations for MEP/zeta potential of protein samples in folded capillary cell |
| 00:23:21 | Conclusions |
| 00:24:09 | Thanks for listening |
| 00:27:13 | Contact Information |
The Zetasizer Nano uses a new diffusion barrier method to protect proteins during a measurement of their charge. The presentation outlines the method and presents a number of application examples