| 00:00:00 | Enhanced Precision Multi Angle DLS |
| 00:01:06 | Poll Question |
| 00:01:06 | Enhanced Precision Multi Angle DLS |
| 00:03:47 | Poll Answers |
| 00:03:47 | Enhanced Precision Multi Angle DLS |
| 00:04:48 | Brownian motion |
| 00:06:47 | Dynamic light scattering (DLS) |
| 00:07:29 | Brownian motion |
| 00:07:37 | Dynamic light scattering (DLS) |
| 00:09:02 | Correlogram deconvolution |
| 00:10:02 | Particle Size Distribution |
| 00:11:05 | Intensity to mass transform |
| 00:13:04 | DLS Results |
| 00:14:23 | Limitations – Experimental Noise |
| 00:17:24 | Intensity to mass transform |
| 00:18:25 | Limitations – Mie Scattering effects |
| 00:22:51 | Inherent Polydispersity |
| 00:24:14 | DLS Precision – Resolution Limits |
| 00:26:48 | Compounding the uncertainty |
| 00:28:11 | Intensity to mass transform |
| 00:28:29 | Compounding the uncertainty |
| 00:30:33 | Historical solution |
| 00:32:33 | Historical Solution – Ideal Samples |
| 00:34:54 | Historical solution – real samples |
| 00:36:45 | The challenge |
| 00:38:15 | The Solution |
| 00:42:19 | Adaptive Correlation |
| 00:45:43 | Multi Angle correlogram collection |
| 00:47:26 | enhanced Resolution Fitting |
| 00:48:36 | Enhanced precision size distribution |
| 00:49:59 | MADLS Example – real samples |
| 00:54:04 | MADLS – Methodology |
| 00:55:56 | Plus – particle concentration |
| 00:56:38 | Particle concentration |
| 00:58:12 | concentration – AAVs |
| 00:59:22 | Concentration – Nanoparticles |
| 01:00:29 | Particle concentration – limitations |
| 01:04:50 | Particle concentration |
| 01:07:48 | Particle concentration – range |
| 01:09:40 | Thank you for your attention |
| 01:09:50 | Question & Answer SessionAsk your question by typing within the Q & A chat facility Listening on-demand ?Send your questions to:events@malvernpanalytical..co |
| 01:11:34 | Particle concentration – limitations |
| 01:14:50 | Question & Answer SessionAsk your question by typing within the Q & A chat facility Listening on-demand ?Send your questions to:events@malvernpanalytical..co |
Dynamic light scattering (DLS) is a common laboratory technique routinely used for sizing proteins, nanoparticles, polymers, & colloidal emulsions. By utilizing the improved accuracy and resolution of multi-angle dynamic light scattering (MADLS®), it is now possible to determine the absolute particle concentration distribution using the Zetasizer Ultra. This provides the user with a fast assessment of the concentration of each population of particles present within their dispersion. In this webinar we will give an overview of the particle concentration analysis on the Zetasizer Ultra and provide some examples for liposomes and virus particles.