Power in numbers - Learn more about your protein's behavior and stability in formulation by adding light scattering functionality to your SEC system

Inicie sesión para ver este seminario web

¿Olvidó su contraseña?
¿Aún no estás registrado? Crea una cuenta
00:00:00 Welcome
00:00:57 "Power in numbers" - Learn more about your protein's behaviour and stability in formulation by adding light scattering functionality to your SEC system.
00:03:22 Proteins
00:04:26 Size Exclusion Chromatography (SEC)
00:05:29 Conventional SEC
00:05:42 Conventional SEC
00:07:33 Beta-amylase: Conventional SEC
00:08:18 BSA: Column calibration
00:09:51 Limitation of available standards
00:11:07 Limitations of Conventional SEC
00:13:22 Light Scattering Detectors
00:13:58 Advanced Detection SEC
00:14:30 Malvern SEC range
00:15:12 How does Light scattering Give me Molecular Weight?
00:15:25 Light Scattering Theory
00:15:50 Light Scattering Theory
00:16:45 Isotropic Scatterers
00:17:46 RIGHT-ANGLE LIGHT SCATTERING (RALS)
00:18:14 Anisotropic Scatterers
00:20:06 Multi-Angle Light Scattering (MALS)
00:20:42 Multi-Angle Light Scattering (MALS)
00:22:23 For more information ……
00:22:52 SEC-mals application examples
00:23:01 Oligomerization Vs. Aggregation
00:23:36 BSA molecular weight using MALS
00:25:21 BSA measurements with MALS
00:26:08 BSA: Column calibration vs MALS
00:26:44 Pepsin aggregation
00:27:51 Pepsin aggregation
00:28:33 Immunoglobulin G
00:28:50 IgG molecular weight
00:30:04 IgG – How does sample loading effect the result?
00:31:01 Aprotinin
00:31:56 IgM
00:32:59 IgM - Lambda
00:33:51 IgM Lambda – Angular dependence
00:34:41 IgM – Kappa
00:36:01 Combined SLS & DLS
00:36:49 Introduction
00:38:14 Why Combine SLS and DLS?
00:39:37 Why combine SLS and DLS
00:41:32 Malvern Zetasizer µV
00:43:15 Multiple Analysis Modes
00:45:38 Application examples of zetasiZer µV
00:45:50 DLS in Batch Mode
00:47:55 DLS in Batch Mode
00:49:42 SLS in Batch Mode
00:51:05 Flow Mode SLS and DLS
00:53:14 Flow Mode SLS and DLS
00:55:14 Summary
00:57:36 Thank you
When developing a formulation stability profile for a biotherapeutics, one of the primary techniques used to identify the oligomeric state and presence or absence of aggregates is size exclusion chromatography (SEC). Traditionally, this technique provides a quantitative analysis of a sample by relating the retention volume of peaks present in the chromatogram to a calibration curve providing a basic identification of the relative molecular weight of each of the peaks.