| 00:00:00 | Welcome |
| 00:01:09 | Unsurpassed protein SEC using the latest innovation in advanced detection, OMNISEC |
| 00:01:32 | Outline |
| 00:01:59 | The Importance of Protein Characterization |
| 00:02:38 | The drivers for better Characterization |
| 00:03:54 | Industry: Biopharmaceuticals |
| 00:04:33 | What can Advanced SEC provide scientists? |
| 00:05:07 | What challenges are there in protein characterization? |
| 00:06:38 | How Can we address them? |
| 00:06:43 | What does advanced SEC offer |
| 00:07:20 | The multi-detection pyramid |
| 00:08:35 | Malvern SEC range |
| 00:09:12 | OMNISEC |
| 00:09:32 | OMNISEC |
| 00:09:52 | OMNISEC software v10.0 |
| 00:10:33 | OMNISEC REVEAL technology |
| 00:11:18 | Refractive index |
| 00:12:12 | UV/Vis PDA |
| 00:12:32 | Light scattering |
| 00:14:07 | Light scattering benefits |
| 00:14:37 | Viscometer |
| 00:15:27 | The benefits of integrated multi-detection |
| 00:16:21 | OMNISEC RESOLVE design |
| 00:16:45 | Separation module – OMINSEC RESOLVE |
| 00:17:40 | Separation module – OMINSEC RESOLVE |
| 00:18:24 | Separation module – OMINSEC RESOLVE |
| 00:18:51 | Why is OMNISEC better? |
| 00:19:32 | What are the results? |
| 00:19:40 | Example chromatography - BSA |
| 00:22:14 | Example chromatography - BSA |
| 00:24:11 | Repeat injections of BSA injections |
| 00:26:16 | BSA – typical analysis |
| 00:27:34 | BSA – typical analysis |
| 00:29:46 | Analysis of antibodies |
| 00:29:55 | Conventional or Single detector SEC |
| 00:30:26 | Conventional calibration: IgG |
| 00:30:48 | Overlay of RALS chromatograms – Sheep IgG, Human IgG |
| 00:31:27 | Triple Detection: IgG |
| 00:32:31 | Triple detection: IgG |
| 00:33:08 | Conventional vs Triple |
| 00:33:35 | Thermal Stability of Human IgG |
| 00:34:27 | Thermal stability of IgG – t=0 |
| 00:35:08 | Thermal stability of IgG – 45 minutes at 60oC |
| 00:35:42 | RI overlay of t=0 and 45 minutes at 60 degrees |
| 00:36:27 | Thermal stability of IgG – 135 minutes at 60oC |
| 00:37:24 | Thermal stability of IgG – 60 hours at 60oC |
| 00:38:37 | Thermal stability of IgG – RT 11 days |
| 00:39:25 | The power of light scattering and IV |
| 00:39:51 | Triple detector data: insights from raw data |
| 00:40:12 | Triple detector data: What do the numbers say? |
| 00:41:26 | Summary |
| 00:41:49 | Thank you for listening |
| 00:41:58 | Contact Information |
| 00:44:03 | Find out more about OMNISEC |
During this webinar, we introduces the latest innovations in SEC technology, and discuss how the new OMNISEC system will assist your protein characterization challenges. Focusing on applications, the webinar compares the capabilities of the system with previous products, and discusses how these technical improvements provide superior protein analysis data and results.
Who should watch?
Protein research chemists - Biochemistry, pharmacology, biopharmaceutical
Academics Lab managers, Biopharmaceutical formulation chemists, Biopharmaceutical development scientists
Anyone concerned with protein aggregation, oligomers, or molecular weight