| 00:00:00 | Polymer solution characterization - Part 3. Branching out! - Intrinsically useful views on polymer structure. |
| 00:01:04 | Polymer solution characterization - Part 3. Branching out! - Intrinsically useful views on polymer structure. |
| 00:01:28 | Polymer solution characterisation - Part 3: Branching out!Intrinsically useful views on polymer structure |
| 00:02:33 | Molecular weight summary |
| 00:03:16 | Determining molecular size - summary |
| 00:04:02 | Solution viscosity measurement |
| 00:05:58 | Intrinsic viscosity – [η] |
| 00:08:22 | Intrinsic viscosity – [η] |
| 00:10:17 | Relation between polymer structure and intrinsic viscosity |
| 00:13:13 | How is intrinsic viscosity measured? |
| 00:13:56 | Traditional measurement |
| 00:15:52 | Huggins/Kraemer Plot |
| 00:16:18 | Two-capillary viscometer – Baseline/Load |
| 00:17:30 | Analysis |
| 00:18:32 | Advanced GPC/SEC Configuration |
| 00:19:16 | Four-capillary viscosity detector |
| 00:21:30 | Mark-Houwink relationship |
| 00:22:28 | Linear MH plot example – Polystyrene in THF (NBS706) |
| 00:23:27 | Difference in Mark Houwink a value for high and low molecular weight polystyrene |
| 00:24:23 | Mark-Houwink plots – different polymer types |
| 00:25:54 | Maltodextrin: Same polymer, different branching |
| 00:27:37 | Calculation of branching – Zimm Stockmayer |
| 00:28:57 | Rg measured by MALS |
| 00:30:22 | Angular dependence in PS235k |
| 00:31:25 | Pectin |
| 00:32:04 | Calculation of branching – Zimm Stockmayer |
| 00:33:26 | Comparison of IV and Rg for structure |
| 00:35:00 | Branching analysis – a worked example |
| 00:35:41 | Comparison of NBS 1475 (Linear) and 1476 (Branched) using M-H plot |
| 00:36:55 | Reproducibility determined by Mark Houwink plot |
| 00:38:01 | Determination of LCB – Interactive branching view |
| 00:38:46 | Long-Chain Branching Parameters |
| 00:40:13 | Untitled |
| 00:42:22 | Branching calculation example: Dextran |
| 00:43:39 | Dextran - branching frequency comparison |
| 00:44:57 | Summary |
| 00:45:36 | Branching out! |
| 00:49:29 | Contact Information |
Most scientists can easily understand polymer molecular weight differences but often intrinsic viscosity, a harder concept to grasp, is a more useful measure of a polymer in solution. This webinar describes in simple terms what intrinsic viscosity is, how it can be measured and why it is the best way to assess the structure and quantify the branching of polymers in solution by GPC/SEC. We include a clear comparison between structural information from radius of gyration and intrinsic viscosity measurements, including how branching number and frequency are calculated. The talk is illustrating with worked examples of both synthetic polymers such as polyethylene and polysaccharides such as hyaluronic acid.