| 00:00:00 | Protein applications for tetra detection. Why upgrade from calibration against protein standards? |
| 00:01:37 | The Benefits of Tetra Detection for Protein Characterisation |
| 00:02:42 | GPC or SEC separates macromolecules in solution |
| 00:04:43 | Conventional Calibration |
| 00:07:41 | Limitations of conventional SEC |
| 00:08:47 | Less compact protein |
| 00:10:44 | Non-globular protein |
| 00:12:12 | Proteasome 20S filled with Cytochrome C |
| 00:14:10 | Fractionation |
| 00:15:46 | Hardware for Tetra Detection |
| 00:17:05 | Tetra Detection |
| 00:18:03 | UV Detection |
| 00:18:34 | RI Detection |
| 00:19:33 | Light Scattering detection |
| 00:21:17 | RALS/LALS Combination |
| 00:25:09 | Intrinsic Viscosity 4- capillary Differential Viscosity Detector |
| 00:27:13 | Typical Tetra Detector Chromatogram |
| 00:28:44 | Tetra Detection |
| 00:29:10 | Molecular weight measurements |
| 00:29:37 | Standard Protein – Bovine Serum Albumin |
| 00:31:33 | Standard Protein – Bovine Serum Albumin |
| 00:31:42 | Oligomer determination |
| 00:32:50 | Proteasome 20S filled with Cytochrome C |
| 00:33:10 | Protein Aggregation |
| 00:33:37 | Cases |
| 00:33:56 | FDA & USP |
| 00:34:13 | Aggregate quantification |
| 00:35:46 | Conformational changes |
| 00:36:15 | Conformational changes |
| 00:38:10 | Antibody analysis |
| 00:38:33 | Protein Conjugation |
| 00:39:22 | Co-polymer analysis |
| 00:40:06 | PEGylated Protein |
| 00:41:06 | Conjugation analysis |
| 00:41:56 | Conjugation analysis |
| 00:42:10 | Summary |
| 00:43:07 | Contact Information |
There are a number of calibration techniques using protein standards for measuring protein molecular weight, the most common being conventional calibration. Why should you upgrade to add light scattering and viscosity detectors?