| 00:00:00 | Mastering Molecular Weight Measurements With The Zetasizer Nano System |
| 00:00:55 | Malvern Instruments Mastering Molecular Weight Measurements With The Zetasizer Nano System From Lab To Line |
| 00:02:31 | Seminar Outline |
| 00:04:13 | Zetasizer Nano System |
| 00:05:26 | Zetasizer Nano Technologies |
| 00:06:19 | Light Scattering Return |
| 00:07:22 | Common Light Scattering Applications |
| 00:09:03 | Light – Matter Interactions |
| 00:11:00 | Intensity vs. Time |
| 00:11:52 | Average Scattering Intensity |
| 00:13:32 | Angle Dependent Scattering |
| 00:14:31 | Classical MW Measurements |
| 00:15:19 | Debye Limit |
| 00:16:06 | Summary & Dilemma |
| 00:16:37 | %Error In The Molecular Weight |
| 00:17:24 | Rg Dependence |
| 00:18:14 | What is Rg? |
| 00:18:57 | Lysozyme - Comparison Of Radii |
| 00:19:56 | DH Dependence |
| 00:20:05 | What is Rg? |
| 00:20:28 | DH Dependence |
| 00:21:55 | System Comparison |
| 00:22:59 | Debye Plot – Globular Proteins |
| 00:24:10 | Debye Plot - Random Coil Polymers |
| 00:24:45 | What Is MW? |
| 00:25:47 | The 2nd Virial Coefficient (A2) |
| 00:27:07 | Second Virial Coefficient (A2)? |
| 00:29:34 | Using A2 As A Stability Predictor |
| 00:30:59 | A2 and Crystallization Success Rate |
| 00:32:26 | A2 Crystallization Window |
| 00:33:33 | Extending The Rayleigh Limit? |
| 00:36:31 | Experimental Procedures |
| 00:38:56 | Is My Sample Suitable For SLS? |
| 00:41:13 | Is My Analyte Suitable For SLS? |
| 00:42:18 | Solvents, Dispersants, & Diluents |
| 00:43:24 | What Is dñ/dC? |
| 00:45:47 | How Important Is dñ/dC? |
| 00:47:05 | How Robust Is dñ/dC? |
| 00:50:32 | Tips – Solvent & Reference |
| 00:55:16 | What About Sample Concentration? |
| 00:57:21 | Can I Estimate Concentration? |
| 00:59:42 | Tips – Sample Prep & Measurement |
| 01:01:38 | What About Dust? |
| 01:02:53 | How Do I Recognize Good Data? |
| 01:05:06 | Data Quality Limits |
| 01:07:10 | Debye Plot Calculator |
| 01:09:18 | Do I Have To Use Toluene? |
| 01:09:55 | Zetasizer MW Specifications |
| 01:11:18 | Thank You |
| 01:12:42 | Contact information |
The Zetasizer Nano software integrates a Debye plot to simplify the measurement of molecular weight. However the biggest source of error is always in sample preparation and handling. This presentation provides training for these measurements