| 00:00:00 | Qualitative and quantitative analysis of proteins using a combination of light scattering techniques in your workflow |
| 00:02:18 | Qualitative and quantitative analysis of proteins using a combination of light scattering techniques in your workflow |
| 00:02:42 | Pharmaceutical industry |
| 00:03:34 | Pharmaceutical Drug Development Pipeline |
| 00:04:56 | Where can light scattering be used? |
| 00:05:59 | Complementary techniques |
| 00:07:10 | Light Scattering |
| 00:07:59 | Static Light Scattering – Molecular Weight |
| 00:08:57 | Hardware for Analytical SEC |
| 00:10:25 | Analytical SEC Chromatogram |
| 00:11:13 | Advanced analytical SEC |
| 00:11:42 | Dynamic Light Scattering |
| 00:13:07 | Correlation Functions |
| 00:14:05 | Size Data |
| 00:15:58 | Instruments and Applications |
| 00:16:37 | Summary of DLS and SEC – pros and cons |
| 00:18:38 | Molecular weight and oligomeric state |
| 00:20:32 | Conformational changes |
| 00:22:02 | Membrane Protein |
| 00:23:58 | Aggregate quantification |
| 00:25:37 | Effect of Storage Conditions |
| 00:27:23 | Oligomeric States of Thermophile protein using DLS |
| 00:28:58 | Protein stabilised by ligand binding |
| 00:29:29 | Measuring Mw and A2 by static light scattering |
| 00:30:57 | Electrophoretic light scattering (Zeta potential) |
| 00:32:54 | Which technique is appropriate? |
| 00:34:53 | Aggregate detection |
| 00:35:32 | Aggregated BSA experiments |
| 00:35:56 | Aggregated BSA - DLS experiments |
| 00:37:52 | Aggregated BSA - DLS experiments |
| 00:40:12 | Aggregated BSA experiments – SEC LS |
| 00:41:55 | Aggregated BSA experiments |
| 00:42:37 | Qualitative aggregate detection |
| 00:43:47 | Oligomer ratio experiments |
| 00:44:29 | Oligomer resolution |
| 00:46:03 | Oligomer resolution |
| 00:47:29 | Formulation |
| 00:48:23 | Formulation |
| 00:49:28 | Summary |
| 00:50:19 | Conclusions |
| 00:51:05 | Contact Information |
Light scattering and chromatography are key tools for protein formulation and characterization studies providing complementary information on protein stability, aggregation, thermal stability, molecular weight, oligomeric state and structure