Size exclusion chromatography (SEC or SEC-HPLC) for measurement of protein absolute molecular weight, structure, size and conformation
Size Exclusion Chromatography (SEC or SEC-HPLC) is an analytical technique that separates dissolved macromolecules by size based on their elution from columns filled with a porous gel.
When SEC is coupled with light scattering, viscometer and concentration detectors (known as triple detection), it can measure absolute molecular weight, molecular size, and intrinsic viscosity and generate information on macromolecular structure, conformation, aggregation and branching.
For polymer and protein characterization, in polymer, food, pharmaceutical and biopharmaceutical research, Malvern Panalytical offers a range of SEC systems or individual SEC detectors, including SEC-MALS, to suit your application needs.
OMNISECThe world’s most advanced multi-detector GPC/SEC system |
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|---|---|
| Technology | |
| Size Exclusion Chromatography (SEC) | |
| Gel Permeation Chromatography | |
| Measurement type | |
| Molecular structure | |
| Molecular size | |
| Molecular weight |
