OMNISEC REVEAL ULTRA

The ultimate in integrated advanced detection for UPLC-SEC

Optimized multi-detector platform designed to work with UPLC-SEC separations. Bring advanced detection measurements of molecular weight, structure and numerous other parameters to the improved resolution of UPLC/UHPLC and APC™ separations. 

It can be added to any existing conventional UPLC system, such as Waters ACQUITY APC™, to offer comprehensive molecular characterization of synthetic and natural polymers, as well as proteins. 

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Overview

OMNISEC REVEAL ULTRA is designed to bring integrated advanced detection to the high resolution separations offered by UPLC-SEC. This unique system improves sample resolution, increases system productivity, reduces sample and solvent consumption. 

Use the OMNISEC REVEAL ULTRA to study the impact of protein oligomerization, aggregation and conjugation on activity and efficacy in academic research and biopharmaceutical development.  

Relate a polymer’s molecular properties to its bulk characteristics in a wide range of applications, from additive manufacturing, to paint and coatings industries to the manufacturing of food additives and pharmaceutical excipients. 

The ultimate in advanced detection for UPLC/UHPLC and APC separations

  • OMNISEC REVEAL ULTRA is compatible with UPLC/UHPLC and APC separations. 
  • Whether you want to perform UPLC-SEC, HPLC-SEC or both the REVEAL ULTRA can perform for you. Enabling method transfer from HPLC to UPLC using the same system, or for future proofing purchases. 
  • Complete flexibility in detector configuration. Only purchase the detectors which add value to you now, with the security that any detector you require in the future can be installed onsite.

Increased productivity, improved resolution, reduced sample consumption, reduced solvent usage

  • Faster run times mean 3 times the productivity from a single system!
  • Improved resolution allows you to see features hidden in standard HPLC-SEC systems
  • Up to 20 times reduction in sample loadings – saving precious samples.  
  • Consume up to 4 times less solvent – reducing disposal costs. 

More reliable measurements with market leading light scattering sensitivity

  • Measure low concentration samples with a highly sensitive and stable refractive index detector
  • Measure molecular weight of polymers as low as 200 g/mol!
  • Incredibly low injection masses for characterization by UPLC-SLS
  • 5ng PS105k in THF
  • 40ng BSA in PBS
  • Make sure you don’t miss low molecular weight and low concentration contaminants or protein fragments 

Multi-detection SEC offers unparalleled information on the characterization of polymers, polysaccharides and proteins

  • Measuring absolute molecular weight, intrinsic viscosity, hydrodynamic radius, Mark-Houwink parameters, radius of gyration, composition, conformation and more
  • Rely on OMNISEC to help you understand, control and optimize your product performance
  • Detection and quantification of protein aggregation to drive faster sample characterization and development

Fully integrated temperature-controlled detector module for improved detector stability and sample characterization

  • Improve separations and result accuracy with the column oven and temperature-controlled integrated detector compartment – all installed as standard! 
  • Minimized inter-detector tubing, maintained at the same temperature as the detector, reduces band broadening effects to further increase signal quality

Improved structural comparisons enabled with the unique mechanical auto-balance of the differential viscometer

  • Boost lab productivity and reduce service costs with a novel self-balancing viscometer design with user exchangeable capillaries

See the full range of your samples UV absorbance with the UV-PDA

  • Distinguish chemical changes by measuring absorbance across the full UV/Vis spectrum (190-900nm) using the photodiode array
  • All UV channels are recorded giving you the flexibility to choose what wavelength to use in post-processing

All systems can be fully serviced and supported onsite

  • No need to worry about shipping systems for service or support, all units can be fully serviced on site to ensure minimum downtime

How it works

Following separation of the sample according to its hydrodynamic volume the sample will pass through 1 or more detectors where its presence is recorded. OMNISEC REVEAL is a fully integrated multi-detection module which can contain up to 4 different detectors. 

The RI and UV detectors give information about the concentration of the components in the sample. The light scattering detectors respond to the intensity of light scattered by the sample which is related to molecular weight and also allows Rg of large molecules to be calculated. The viscometer measures the changing solution viscosity to calculate the intrinsic viscosity of the sample which is a measure of structure. 

Coupling the efficient sample separation of OMNISEC RESOLVE with the fully optimized, multi-detector, information-rich analysis delivered by OMNISEC REVEAL, Malvern Panalytical has produced a market-leading solution for researchers and formulators developing novel natural and synthetic polymers, pharmaceutical and biopharmaceutical products and foods.

05_RI

Refractive Index

  • Measure the concentration of any solute with excellent sensitivity
06_UVvisPDA

UV/Vis PDA

  • Measure absorbance at any wavelength from 190-900 nm
  • Assess changes in absorbance spectra
  • Characterize copolymers
07_LightScattering

Light Scattering

  • Measure absolute molecular weight in a wider range of applications
  • Measure as little as 100 ng of material
  • Measure molecular weights down to 200 Da
08_Viscometer

Viscometer

  • Better intrinsic viscosity measurements thanks to automated balancing
  • Reduced service cost and time thanks to user-exchangeable capillaries

Specification

System

Dimensions (W, D, H) 420, 640, 600 mm
Weight 40 kg
Detector temperature range 20 - 65 °C
Software OMNISEC software v10 (or later)
Data acquisition rate 100 Hz
Patents

US20140060162A1 & EP2619543B1 "Modular capillary bridge viscometer"

US20140144214A1 & EP2619544A1 "Automatically balanced capillary bridge viscometer"

Detector 1

Detector Light scattering
Measurement principle RALS 90° angle, LALS 7° angle
Light source 50 mW, 640 nm laser
Cell volume 18 µL
Dynamic range 2500 mV
Baseline noise <0.1 mV
Baseline drift <0.2 mV/hr

Detector 2

Detector Differential refractive index
Measurement principle Deflection
Cell volume 12 µL
Dynamic range ±2.5 x 10-4 RIU
Baseline noise <10-7 RIU
Baseline drift <3 x 10-7 RIU/hr

Detector 3

Detector Viscometer
Measurement principle 4-capillary Wheatstone bridge with self-balancing mechanism and user-exchangeable capillaries
Detector volume 17 µL/capillary
Differential pressure dynamic range ±5000 Pa
Differential pressure baseline noise <0.3 Pa
Inlet pressure dynamic range 100 kPa
Inlet pressure baseline noise 0.01 kPa
Baseline drift <0.2 kPa/hr

Detector 4

Detector Diode-array-based UV/Vis spectrometer
Wavelength 190 - 900 nm
Number of wavelengths 1024
Wavelength accuracy <1 nm
Wavelength resolution 0.6 nm
Cell volume 7.5 µL
Path length 10 mm
Baseline noise 2 x 10-5 AU
Baseline drift 5 x 10-4 AU/hr

Measurement type 1

Measurement type Absolute molecular weight
Measurement range 200 - >107 g/mol
Minimum quantifiable mass Using UPLC-SEC:
5 ng of 100 kDa molecular weight polystyrene in THF
40 ng of BSA in PBS
Measurement principle Light scattering (LALS or RALS)

Measurement type 2

Measurement type Intrinsic viscosity
Minimum quantifiable mass Using UPLC-SEC:
100 ng of 100 kDa molecular weight polystyrene in THF
Measurement principle 4-capillary Wheatstone bridge

Measurement type 3

Measurement type Concentration
Minimum quantifiable mass
Using UPLC-SEC:
5 ng of 100 kDa molecular weight polystyrene in THF
40 ng of BSA in PBS
Measurement principle Differential refractive index detection

Operating environment

Temperature 15 °C - 30 °C