Overview
Malvern Panalytical offers a full range of analytical GPC/SEC columns for a wide range of applications and molecular weights. These include common column types for synthetic polymers in organic solvents as well as water soluble polymers (e.g. polysaccharides and DNA). Silica-based columns for separation of proteins are also available. Finally, Malvern Panalytical offers a range of speciality columns for running in polar solvents or with cationic polymers.
- Styrene-divinyl benzene (SDVB) columns for organic soluble synthetic polymers
- SDVB columns shipped in THF
- Methacrylate columns for water soluble polymers and polysaccharides
- Silica columns for separations of proteins
- Columns for polymers soluble in polar solvents
- Columns for strongly cationic samples such as chitosan
- Single and mixed pore sizes to maximize molecular weight resolution or linearity
- Guard columns for all applications
How it works
Polymer molecules dissolved in the mobile phase pass through the GPC/SEC column. As they move along the column, the sample molecules enter and leave pores in the column matrix. Larger molecules are excluded from more pores and thus travel faster through the column and elute sooner. Smaller molecules enter more pores and therefore progress is slower so they elute later.
Molecules are thus separated by their hydrodynamic size and measured by one or more detectors when they elute from the column.
Specification
Styrene-divinyl benzene (T-series)
Column type | Styrene-divinyl benzene (T-series) |
---|---|
Applications | Synthetic polymers |
Shipping solvents | THF |
Exclusion limits | Exclusion limits (Da Polystyrene)
|
Compatible solvents | THF, Chloroform, Toluene, Xylene, Acetone, MEK, DMF, DMAc, HFIP, NMP, and others. For a full list, request or download the manual. |
Dimensions | 300 mm x 8.0 mm |
Polymethyl-methacrylate (A-series)
Column type | Polymethyl-methacrylate (A-series) |
---|---|
Applications | Water soluble polymers |
Shipping solvents | Water |
Exclusion limits | Exclusion limits (Da Pullulan) Single-pore columns: 4,000 (A2000) 10,000 (A2500) 100,000 (A3000) 1,000,000 (A4000) 4,000,000 (A5000) 20,000,000 (A6000) 500,000,000 (A7000) General-purpose mixed-bed: 20,000,000 (A6000M) |
Compatible solvents | Aqueous buffers with organic modifiers such as acetonitrile, methanol, DMF and DMSO. |
Dimensions | 300 mm x 8.0 mm |
Silica (P-, PLS-series)
Column type | Silica (P-, PLS-series) |
---|---|
Applications | Proteins |
Shipping solvents | Water |
Exclusion limits | Exclusion limits (Da Protein):
150k (P2500) 700k (P3000) 1,000k (P4000) 1,250k (PLS5030, PLS3030, PLS3030H) |
Compatible solvents | Aqueous buffers with organic modifiers such as acetonitrile, methanol and ethanol. |
Dimensions | 300 mm x 8.0 mm |
pH range | 3.0 – 7.5 |