| 00:00:00 | The Zetasizer Ultra - A novel assay for measuring Adeno-Associated Virus (AAV) |
| 00:02:21 | Agenda |
| 00:03:22 | Section 1:Introduction to the Zetasizer Ultra and MADLS |
| 00:03:41 | Zetasizer – Introduction and Overview |
| 00:06:37 | Zetasizer – Key Technology |
| 00:08:04 | Zetasizer Ultra – What's new? |
| 00:10:15 | Measurement Principle - Dynamic Light Scattering (DLS) |
| 00:12:10 | Limitations of Single Angle DLS |
| 00:14:11 | Measurement Principle - Multi Angle Dynamic Light Scattering (MADLS) |
| 00:15:37 | Zetasizer Ultra - Particle Concentration |
| 00:16:53 | Section 2: Adeno-Associated Virus in Gene Therapy development |
| 00:17:13 | Adeno-Associated Virus – Gene Therapy Vector |
| 00:19:38 | Adeno-Associated Virus – Structure |
| 00:19:38 | Adeno-Associated Virus – Common Titer Assays |
| 00:19:38 | Adeno-Associated Virus – Zetasizer Ultra Assays |
| 00:21:06 | Section 3: AAV Example Data |
| 00:21:06 | Zetasizer Ultra – Example AAV Data |
| 00:21:32 | Case Study #1: Application Note |
| 00:22:38 | Case Study #1: Application Note |
| 00:23:38 | Case Study #1: Application Note |
| 00:24:51 | Case Study #2: AAV Thermal Stability Test |
| 00:26:37 | Case Study #2: Particle Concentration by MADLS |
| 00:28:13 | Case Study #2: In-Process AAV Characterization |
| 00:30:21 | Section 4: Building a MADLS Method for AAV |
| 00:30:32 | ZS Explorer Software |
| 00:30:53 | Particle Concentration – How it Works |
| 00:33:13 | Zetasizer Ultra – Method parameters |
| 00:37:39 | Zetasizer Ultra – Summary and Conclusions |
| 00:39:17 | Questions |
| 00:40:13 | Thanks for listening |
The high-resolution size capabilities of the Zetasizer Ultra have enabled a new assay to rapidly characterize Adeno Associated Virus (AAV) viral concentration. Utilizing Multi Angle Dynamic Light Scattering (MADLS), the Zetasizer Ultra can determine both AAV viral concentration and size in a single measurement that is cuvette-based, rapid, label-free, low volume, and non-destructive. In this talk, we present AAV viral concentration and size results, the MADLS measurement principle, and the effect of sample properties, such as material refractive index and viscosity.
Speakers
Jonathan Mehtala - Field Applications Scientist - Malvern Panalytical
More information
- Who should attend
- Anyone working with AAV
- Those interested in novel applications of dynamic light scattering
- Those interested in sample characterization using MADLS
- Anyone who would like to understand more about MADLS and the Zetasizer Ultra
- What will they learn
- How MADLS can be used to perform size and concentration analysis of samples including AAV