Hyaluronic acid (HA) is a natural polysaccharide, that plays an important role in tissues, such as skin, tendons, muscles, and cartilage. As well as medical applications, It finds wide-spread use in cosmetics, particularly skin creams. In this presentation, we demonstrate how a suite of combined analytical techniques can give insight into the structural difference that affect the efficacy of the final products. The information revealed includes molecular size and charge, absolute molecular weight distribution, branching structure and rheological properties.