| 00:00:00 | From the applications laboratory What can GPC do for me? |
| 00:02:03 | From The Application Lab – What Can GPC Do For Me? |
| 00:02:23 | Abstract |
| 00:03:04 | Outline |
| 00:04:01 | Defining Some Terms |
| 00:05:01 | Defining Some Terms |
| 00:06:39 | What is Chromatography? |
| 00:07:26 | Size Exclusion Chromatography |
| 00:09:10 | The Separation Process |
| 00:10:46 | Why Use Size Exclusion Chromatography? |
| 00:12:37 | Molecular Weight Moments |
| 00:13:25 | Molecular Weight Moments – Distribution Averages |
| 00:14:19 | Understanding Your Sample |
| 00:16:22 | Components of an SEC System |
| 00:18:23 | Components of an SEC System |
| 00:18:32 | SEC/GPC Data Processing Options |
| 00:19:29 | Conventional Calibration |
| 00:19:39 | Conventional Calibration – Detectors from Viscotek |
| 00:20:42 | Concentration Detectors |
| 00:21:17 | Creating a Calibration Curve |
| 00:22:07 | Conventional GPC Systems |
| 00:22:32 | Overlay of Conventional Calibration Curves |
| 00:23:18 | Conventional Calibration |
| 00:24:13 | Conventional Calibration - Summary |
| 00:24:53 | Universal Calibration |
| 00:25:12 | Four-Capillary viscometer |
| 00:26:03 | Universal Calibration |
| 00:27:10 | Universal Calibration – PMMA 64 kDa Narrow Standard |
| 00:28:31 | Universal Calibration Conclusions |
| 00:29:40 | Triple Detection |
| 00:29:55 | Static Light Scattering – Rayleigh Theory |
| 00:30:23 | Static Light Scattering Summary |
| 00:31:14 | Triple Detection |
| 00:33:30 | Process Prediction Based on Structure |
| 00:34:54 | Long-Chain Branching |
| 00:35:16 | How is LCB Determined? |
| 00:36:11 | Structural Differences in Cellulose Excipients |
| 00:37:13 | Density Changes in CPVC -QA/QC |
| 00:38:06 | Applications – Variables To Consider |
| 00:40:04 | Aminated Chitosan – Cationic Column Selection |
| 00:40:45 | Polyalkylacrylamide - Inert Column Selection |
| 00:41:33 | PET in HFIPA – Inert Column Selection |
| 00:42:28 | HPMC in Acetic Acid – Glycosylated Column Selection |
| 00:43:26 | Polycarbonate |
| 00:45:14 | Functionalizing Chitosan |
| 00:46:01 | Functionalizing Chitosan |
| 00:46:32 | HT-GPC Applications |
| 00:46:37 | Polymers For High Temperature GPC/SEC |
| 00:47:12 | Polyethylene (PE) and Polypropylene (PP) |
| 00:48:19 | PE and PP at 140 °C in TCB |
| 00:49:36 | PP at 160 °C in TCB |
| 00:51:29 | Protein Analysis |
| 00:51:36 | Intrinsic Viscosity for Proteins |
| 00:52:12 | Tetra Detection GPC for Proteins - BSA |
| 00:52:47 | BSA – Molecular Weight Determination of Complexes |
| 00:53:31 | Untitled |
| 00:54:06 | BSA Aggregation – Chromatography Temp. = 45 °C |
| 00:54:43 | Compositional Analysis |
| 00:55:34 | PEGylated Proteins – Compositional Analysis |
| 00:56:08 | Untitled |
| 00:56:41 | Conclusions |
| 00:58:04 | Contact Information |
This overview of GPC/SEC gives examples of polyolefins, proteins and biopolymers that benefit from this powerful analytical tool. Applications range from standard macromolecular characterization to advanced triple detection